Rhesus IL-18 (His Tag) recombinant protein

Rhesus IL-18 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Interleukin-18 (IL-18, also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of IL-18 and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL-18 is an IL-1−like cytokine that requires cleavage with caspase-1 to become active, was found to increase IgE production in a CD4+ T cells-, IL-4− and STAT6−dependent fashion. IL-18 and T cell receptor−mediated stimulation could induce naïve CD4+ T cells to develop into IL-4−producing cells in vitro. Thus, caspase-1 and IL-18 may be critical in regulation of IgE production in vivo, providing a potential therapeutic target for allergic disorders. IL-18 production in primary synovial cultures and purified synovial fibroblasts was, in turn, upregulated by TNF-α and IL-1β, suggesting that monokine expression can feed back to promote Th1 cell development in synovial membrane. Besides, synergistic combinations of IL-18, IL-12, and IL-15 may be of importance in sustaining both Th1 responses and monokine production in RA.

IL-1G; MGC12320; IL18; IL-18; IGIF; IL1F4

Q9BGI5

E. coli

A DNA sequence encoding the rhesus IL-18 (NP_001028006.1) (Ala 2-Asp 193) was expressed with an N-terminal polyhistidine tag.

Rhesus

The recombinant rhesus IL18 consists of 203 amino acids and has a calculated molecular mass of 23.7 kDa.

> 92 % as determined by SDS-PAGE

1. Measured by its ability to bind human IL18R1 in a functional ELISA.
2. Measured by its ability to induce IFNγsecretion by KG-1 human acute myelogenous leukemia cells in the presence of TNFα. The ED₅₀ for this effect is typically 3-15 μg/mL in the presence of 20 ng/mL recombinant human TNFα.