Product Name

Pre-IL18 antibody

Documents

Description

Pre-IL18 Mouse Monoclonal antibody. Positive WB detected in HeLa cells, HepG2 cells. Positive IF detected in MCF-7 cells, Hela cells. Observed molecular weight by Western-blot: 24 kDa

Tested applications

ELISA, IF, WB

Species reactivity

Human, hamster; other species not tested.

Alternative names

IbOCtadekin antibody; IFN gamma inducing factor antibody; IGIF antibody; IL 1 gamma antibody; IL 18 antibody; IL 1g antibody; IL18 antibody; IL1F4 antibody; Interleukin 1 gamma antibody; Interleukin 18 antibody

Isotype

Mouse IgG2b

Preparation

This antibody was obtained by immunization of Pre-IL18 recombinant protein (Accession Number: NM_001386420). Purification method: Caprylic acid/ammonium sulfate precipitation.

Clonality

Monoclonal

Formulation

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IF: 1:10-1:100

Validations

HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107484(Pre-IL18 Antibody) at dilution of 1:1000

Immunofluorescent analysis of MCF-7 cells, using IL18 antibody Catalog No: at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).

Immunofluorescent analysis of Hela cells, using IL18 antibody Catalog No: at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).

Background

IL18, is a proinflammatory cytokine involved in the development of Th1 cells and in immune response. It can stimulate the NK cells and certain T cells to release interferon gamma which plays an important role in activating the macrophages or other cells. IL18 has been demonstrated to have the potential to enhance Fas ligand-mediated cytotoxicity, which is increased in PE and regulates placental apoptosis. IL18 is synthesized as a 24 kDa precursor and then cleaved into a biologically activate 18 kDa form.

Pre-IL18 antibody

Product Information

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