Human IL-18 recombinant protein

Human IL-18 recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Interleukin-18 (IL-18, also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of IL-18 and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL-18 is an IL-1−like cytokine that requires cleavage with caspase-1 to become active, was found to increase IgE production in a CD4+ T cells-, IL-4− and STAT6−dependent fashion. IL-18 and T cell receptor−mediated stimulation could induce naïve CD4+ T cells to develop into IL-4−producing cells in vitro. Thus, caspase-1 and IL-18 may be critical in regulation of IgE production in vivo, providing a potential therapeutic target for allergic disorders. IL-18 production in primary synovial cultures and purified synovial fibroblasts was, in turn, upregulated by TNF-α and IL-1β, suggesting that monokine expression can feed back to promote Th1 cell development in synovial membrane. Besides, synergistic combinations of IL-18, IL-12, and IL-15 may be of importance in sustaining both Th1 responses and monokine production in RA.

Interleukin-18

MGC12320; IL18; IL-1G; IL1F4; IGIF; IL-18

Q14116

IL18; IGIF; IL1F4

E. coli

A DNA sequence encoding the human IL18 (NP_001553.1) (Tyr37-Asp193) was expressed.

Human

The recombinant human IL18 consists 157 amino acids and predicts a molecular mass of 18.2 kDa.

>90 % as determined by SDS-PAGE. >90 % as determined by SEC-HPLC.

Measured by its ability to induce IFN-gamma secretion by KG‑1 human acute myelogenous leukemia cells in the presence of TNF-alpha. The ED₅₀ for this effect is typically 5-30 ng/ml.