Rat SELP / P Selectin (Fc Tag) recombinant protein

Rat SELP / P Selectin (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

P selectin (SELP) is a 140kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. SELP mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. P selectin is a cell adhesion molecule on the surface of activated endothelial cells. Cellular adhesion molecules are a large family of proteins that attach the cytoskeleton and intracellular signaling cascades with the extracellular environment. SELP is a calcium-dependent receptor for myeloid cells that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interacton of activated endothelial cells or platelets with leukocytes.

Selp protein

LEUKOCYTE-ENDOTHELIAL CELL ADHESION MOLECULE 3; LECAM3; RP1-86F14.2; GMP-140; GRANULE MEMBRANE PROTEIN 140; CD62P; GMP140; PLATELET ACTIVATION DEPENDENT GRANULE-EXTERNAL MEMBRANE PROTEIN; PADGEM; CD62 ANTIGEN-LIKE FAMILY MEMBER P; GRMP; PSEL; CD62; P-SELECTIN

Q3MID1

Selp

Human Cells

A DNA sequence encoding the rat Selp (AAI01920.1) (Met1-Gln700) was expressed with the Fc region of human IgG1 at the C-terminus.

Rat

The recombinant rat Selp consists 897 amino acids and predicts a molecular mass of 98.7 kDa.

> 95 % as determined by SDS-PAGE.

Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells.
When 5 x 10E4 cells/well are added to rat SELP/Fc Chimera coated plates (10 μg/mL with 100 μL/well), > 40% cells will adhere for 1 hour incubation at 37℃.