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Product Name
Anti-SELP / P Selectin antibody
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Description
Rabbit polyclonal to SELP / P Selectin
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Tested applications
ELISA, WB
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Species reactivity
Cynomolgus SELP / P Selectin
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Alternative names
GMP-140 antibody; granule membrane protein 140 antibody; LECAM3 antibody; leukocyte-endothelial cell adhesion molecule 3 antibody; PADGEM antibody; platelet activation dependent granule-external membrane protein antibody; P-selectin antibody; CD62 antibody; CD62 antigen-like family member P antibody; CD62P antibody; GMP140 antibody; GRMP antibody; LECAM3 antibody; PADGEM antibody; PSEL antibody; RP1-86F14.2 antibody; CD62 antibody; GRMP antibody; PSEL antibody; CD62P antibody; GMP140 antibody; LECAM3 antibody; PADGEM antibody; Grmp antibody; CD62P antibody; LECAM3 antibody; PADGEM antibody; GMP-140 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Cynomolgus SELP / P Selectin (rh SELP / P Selectin; XP_001094728.1; Met1-Ala771). SELP / P Selectin specific IgG was purified by Cynomolgus SELP / P Selectin affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 10-15 μg/ml
ELISA:0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Cynomolgus SELP / P Selectin. The detection limit for Cynomolgus SELP / P Selectin is < 0.039 ng/well.
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Validations
SELP / P Selectin Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
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Background
P selectin (SELP) is a 140kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. SELP mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. P selectin is a cell adhesion molecule on the surface of activated endothelial cells. Cellular adhesion molecules are a large family of proteins that attach the cytoskeleton and intracellular signaling cascades with the extracellular environment. SELP is a calcium-dependent receptor for myeloid cells that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interacton of activated endothelial cells or platelets with leukocytes.
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References
- Johnson-Tidey RR, et al. (1994) Increase in the adhesion molecule P-selectin in endothelium overlying atherosclerotic plaques. Coexpression with intercellular adhesion molecule-1. Am J Pathol. 144(5):952-61.
- Walcheck B, et al. (1996) Neutrophil-neutrophil interactions under hydrodynamic shear stress involve L-selectin and PSGL-1. A mechanism that amplifies initial leukocyte accumulation of P-selectin in vitro. J Clin Invest. 98(5):1081-7.
- Foreman KE, et al. (1994) C5a-induced expression of P-selectin in endothelial cells. J Clin Invest. 94(3):1147-55.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"