Rat gp130/CD130 (His Tag) recombinant protein

Rat gp130/CD130 (His Tag) recombinant protein

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Glycoprotein 130 (also known as gp130, IL6ST, IL6-beta or CD130) is a transmembrane protein which is the founding member of the class of all cytokine receptors. CD130/gp130 is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and Oncostatin M (OSM). CD130/gp130 functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. CD130/gp130 plays a critical role in regulating myocyte apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described. A related pseudogene has been identified on chromosome 17. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. CD130/gp130 binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. CD130/gp130 may have a role in embryonic development. The type I OSM receptor is capable of transducing OSM-specific signaling events.

Interleukin-6 receptor subunit beta

IL6R-BETA; IL-6RB; BB405851; CD130; 5133400A03RIK; AA389424; GP130; D13ERTD699E; GP130-RAPS; IL6ST; CDW130

H9BFG4

Il6st; rCG_44468

Human Cells

A DNA sequence encoding the rat IL6ST (NP_001008725.2) extracellular domain (Met 4-Glu 618) was fused with a polyhistidine tag at the C-terminus.

Rat

The secreted recombinant rat IL6ST comprises 607 amino acids and predicts a molecular mass of 68.2 kDa. The apparent molecular mass of the rat IL6ST is approximately 90-100 kDa in SDS-PAGE under reducing conditions due to glycosylation.

> 97 % as determined by SDS-PAGE

Measured by its ability to inhibit the IL6 Rα enhancement of IL6 activity on M1 mouse myeloid leukemia cells (Saito, T. et al. 1991, J. Immunol. 147:168.). The ED₅₀ for this effect is typically 0.5-2 μg/ml in the presence of 50 ng/ml recombinant human IL6sR and 100 ng/ml recombinant human IL6.