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Product Name
Mouse gp130/CD130 (His & Fc Tag) recombinant protein
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Description
Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development.
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Protein name
Interleukin-6 receptor subunit beta
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Protein short names
CDW130; IL6ST; GP130-RAPS; CD130; D13ERTD699E; 5133400A03RIK; AA389424; GP130; IL-6RB; BB405851; IL6R-BETA
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Uniprot ID
Q6PDI9
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Gene Name
Il6st
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Source/Expression Host
CHO Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the mouse gp130 (NP_034690.3) extracellular domain (Met 1-Glu 617) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
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Protein Species
Mouse
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Molecular weight
The recombinant mouse gp130/Fc chimera is a disulfide-linked homodimer. The reduced monomer consists of 843 amino acids and has a predicted molecular mass of 94.7 kDa. As a result of glycosylation, the apparent molecular mass of rm gp130/Fc monomer is approximately 120-130 kDa in SDS-PAGE under reducing conditions.
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Purity
> 95 % as determined by SDS-PAGE
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Activity
Measured by its ability to inhibit the IL6 Rα enhancement of IL6 activity on M1 mouse myeloid leukemia cells (Saito, T. et al. 1991, J. Immunol. 147:168.). The ED50 for this effect is typically 0.03-0.15 μg/ml in the presence of 10 ng/ml recombinant human IL6sR and 20 ng/ml recombinant human IL6.
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Validations
Mouse IL6ST / CD130 Protein (His & Fc Tag) SDS-PAGE
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