-
Product Name
Rat gp130/CD130 (His & Fc Tag) recombinant protein
- Documents
-
Description
Glycoprotein 130 (also known as gp130, IL6ST, IL6-beta or CD130) is a transmembrane protein which is the founding member of the class of all cytokine receptors. CD130/gp130 is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and Oncostatin M (OSM). CD130/gp130 functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. CD130/gp130 plays a critical role in regulating myocyte apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described. A related pseudogene has been identified on chromosome 17. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. CD130/gp130 binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. CD130/gp130 may have a role in embryonic development. The type I OSM receptor is capable of transducing OSM-specific signaling events.
-
Protein name
Interleukin-6 receptor subunit beta
-
Protein short names
IL6ST; CDW130; GP130-RAPS; GP130; AA389424; 5133400A03RIK; D13ERTD699E; CD130; IL6R-BETA; BB405851; IL-6RB
-
Uniprot ID
H9BFG4
-
Gene Name
Il6st; rCG_44468
-
Source/Expression Host
Human Cells
-
Expression Plasmid/cDNA
A DNA sequence encoding the rat IL6ST (NP_001008725.2) extracellular domain (Met 4-Glu 618) was fused with the C-terminal polyhistidine-tagged Fc region of mouse IgG1 at the C-terminus.
-
Protein Species
Rat
-
Molecular weight
The recombinant rat IL6ST/Fc is a disulfide-linked homodimer. The reduced monomer comprises 839 amino acids and predicts a molecular mass of 94.3 kDa. The apparent molecular mass of the rat IL6ST/Fc monomer is approximately 130 kDa in SDS-PAGE under reducing conditions due to glycosylation.
-
Purity
> 95 % as determined by SDS-PAGE
-
Activity
Measured by its ability to inhibit the IL6 Rα enhancement of IL6 activity on M1 mouse myeloid leukemia cells (Saito, T. et al. 1991, J. Immunol. 147:168.). The ED50 for this effect is typically 0.015-0.075 μg/ml in the presence of 50 ng/ml recombinant human IL6sR and 100 ng/ml recombinant human IL6.
-
Validations
Rat gp130 / IL6ST / CD130 Protein (His & Fc Tag) SDS-PAGE
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"