Rat Ephrin-B1/EFNB1 (His Tag) recombinant protein

Rat Ephrin-B1/EFNB1 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Ephrin-B1 also known as EFNB1, is a member of the ephrin family. The transmembrane- associated ephrin ligands and their Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Eph/ephrin interactions are implicated in axon guidance, neural crest cell migration, establishment of segmental boundaries, and formation of angiogenic capillary plexi. Eph receptors and ephrins are divided into two subclasses, A and B, based on binding specificities. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. An exception is the EphA4 receptor, which binds both subclasses of ephrins. EphrinB1 and B class Eph receptors provide positional cues required for the normal morphogenesis of skeletal elements. Another malformation, preaxial polydactyly, was exclusively seen in heterozygous females in which expression of the X-linked ephrinB1 gene was mosaic, so that ectopic EphB-ephrinB1 interactions led to restricted cell movements and the bifurcation of digital rays.

Ephrin-B1

CFND; EPLG2; EFL3; ELK-L; MGC8782; STRA1; EPHRIN B1; EPL2; LERK2; CFNS; RP23-110A15.1; EPHRIN-B1; EFB1; EFL-3; LERK-2; RP3-496G1.1; EFNB1; CEK5-L

P52796

Efnb1; Eplg2; Lerk2

Human Cells

A DNA sequence encoding the rat EFNB1 (P52796) extracellular domain (Met 1-Thr 229) was expressed, fused with a polyhistidine tag at the C-terminus.

Rat

The recombinant rat EFNB1 comprises 216 amino acids and predicts a molecular mass of 23.8 kDa. The apparent molecular mass of the ratEFNB1 is approximately 36 kDa in SDS-PAGE under reducing conditions due to glycosylation.

> 95 % as determined by SDS-PAGE

Immobilized rat EFNB1-His at 10 μg/ml (100 μl/well) can bind biotinylated mouse EphB3-His (Cat:500494), The EC₅₀ of biotinylated mouse EphB3-His (Cat:500494) is 6.3-14.6 ng/ml.