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Product Name
Rat Ephrin-B1/EFNB1 (Fc Tag) recombinant protein
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Description
Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to the receptor tyrosine kinases EPHB3 (preferred), EPHB1 and EPHA1. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons (By similarity).
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Protein name
Ephrin-B1
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Protein short names
EFB1; LERK-2; EFL-3; EPHRIN-B1; RP23-110A15.1; CFNS; LERK2; EPL2; EPHRIN B1; CEK5-L; EFNB1; RP3-496G1.1; EPLG2; CFND; STRA1; MGC8782; ELK-L; EFL3
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Uniprot ID
P52796
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Gene Name
Efnb1; Eplg2; Lerk2
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the rat EFNB1 (P52796) (Met1-Thr229) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
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Protein Species
Rat
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Molecular weight
The recombinant rat EFNB1 comprises 446 amino acids and predicts a molecular mass of 49.4 kDa. The apparent molecular mass of the rat EFNB1 is approximately 58 kDa in SDS-PAGE under reducing conditions due to glycosylation.
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Purity
> 98 % as determined by SDS-PAGE
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Activity
Immobilized mouse EphB3-His (Cat:500494) at 10 μg/ml (100 μl/well) can bind ratEFNB1-Fc, The EC50 of ratEFNB1-Fc is 6.1-14.2 ng/ml.
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Validations
Rat Ephrin-B1 / EFNB1 Protein (Fc Tag) SDS-PAGE
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