Human Ephrin-B1/EFNB1 (His Tag) recombinant protein

Human Ephrin-B1/EFNB1 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Ephrin-B1 also known as EFNB1, is a member of the ephrin family. The transmembrane- associated ephrin ligands and their Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Eph/ephrin interactions are implicated in axon guidance, neural crest cell migration, establishment of segmental boundaries, and formation of angiogenic capillary plexi. Eph receptors and ephrins are divided into two subclasses, A and B, based on binding specificities. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. An exception is the EphA4 receptor, which binds both subclasses of ephrins. EphrinB1 and B class Eph receptors provide positional cues required for the normal morphogenesis of skeletal elements. Another malformation, preaxial polydactyly, was exclusively seen in heterozygous females in which expression of the X-linked ephrinB1 gene was mosaic, so that ectopic EphB-ephrinB1 interactions led to restricted cell movements and the bifurcation of digital rays.

Ephrin-B1

EPL2; EPHRIN B1; EFB1; EFL-3; LERK-2; RP23-110A15.1; EPHRIN-B1; CFNS; LERK2; EFNB1; RP3-496G1.1; CEK5-L; EPLG2; CFND; EFL3; STRA1; MGC8782; ELK-L

P98172

EFNB1; EFL3; EPLG2; LERK2

Human Cells

A DNA sequence encoding the human EFNB1 (NP_004420.1) extracellular domain (Met 1-Gly 232) was fused with a polyhistidine tag at the C-terminus.

Human

The recombinant human EFNB1 consists of 221 amino acids and predicts a molecular mass of 24.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh EFNB1 is approximately 38 kDa due to glycosylation.

> 95 % as determined by SDS-PAGE

1. Measured by its binding ability in a functional ELISA.
2. Immobilized recombinant human EphrinB1 at 10 μg/ml (100 μl/well) can bind human EphB6 with a linear range of 0.16-4 μg/ml.