Mouse Ephrin-B1/EFNB1 (Fc Tag) recombinant protein

Mouse Ephrin-B1/EFNB1 (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Ephrin-B1 also known as EFNB1, is a member of the ephrin family. The transmembrane- associated ephrin ligands and their Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Eph/ephrin interactions are implicated in axon guidance, neural crest cell migration, establishment of segmental boundaries, and formation of angiogenic capillary plexi. Eph receptors and ephrins are divided into two subclasses, A and B, based on binding specificities. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. An exception is the EphA4 receptor, which binds both subclasses of ephrins. EphrinB1 and B class Eph receptors provide positional cues required for the normal morphogenesis of skeletal elements. Another malformation, preaxial polydactyly, was exclusively seen in heterozygous females in which expression of the X-linked ephrinB1 gene was mosaic, so that ectopic EphB-ephrinB1 interactions led to restricted cell movements and the bifurcation of digital rays.

Ephrin-B1

EFL3; STRA1; MGC8782; ELK-L; EPLG2; CFND; EFNB1; RP3-496G1.1; CEK5-L; EPL2; EPHRIN B1; LERK-2; EFB1; EFL-3; EPHRIN-B1; RP23-110A15.1; CFNS; LERK2

Q544L9

Efnb1; Epl2; Eplg2; Lerk2; Stra1

Human Cells

A DNA sequence encoding the mouse EFNB1 (NP_034240.1) extracellular domain (Met 1-Ser 229) was as fused with the Fc region of human IgG1 at the C-terminus.

Mouse

The recombinant mouse EFNB1/Fc is a disulfide-linked homodimer. The reduced monomer consists of 441 amino acids and has a predicted molecular mass of 49 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmEFNB1/Fc monomer is approximately 60 kDa due to glycosylation.

> 85 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA. Immobilized mouse EphB3 at 2 μg/ml (100 μl/well) can bind mouse EFNB1 with a linear range of 0.1-12.5 ng/ml.