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Human R-Spondin 1 / RSPO1 recombinant protein

Cat.#: 502290

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Product Information

  • Product Name
    Human R-Spondin 1 / RSPO1 recombinant protein
  • Documents
  • Description
    Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination.
  • Protein name
    R-spondin-1
  • Protein short names
    RSPONDIN; RSPO1; R-SPONDIN; CRISTIN3; RP23-325M14.2; R-SPONDIN1; RP11-566C13.1; RSPO; FLJ40906
  • Uniprot ID
    Q2MKA7
  • Gene Name
    RSPO1
  • Source/Expression Host
    CHO Cells
  • Expression Plasmid/cDNA
    A DNA sequence encoding the human RSPO1 (NP_001033722.1) ( Met 1-Ala 263 )was expressed and purified.
  • Protein Species
    Human
  • Molecular weight
    The recombinant human RSPO1 consists of 243 amino acids and has a predicted molecular mass of 26.8 kDa. As a result of glycosylation, the apparent molecular mass of it is approximately 40 and 31 kDa in SDS-PAGE under reducing conditions.
  • Purity
    (53.2+43.4) % as determined by SDS-PAGE
  • Activity
    Measured by its ability to induce activation of ßcatenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells.
    The ED50 for this effect is typically 40-400 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt3a.
  • Validations

    Human R-Spondin 1 / RSPO1 Protein SDS-PAGE

    Human R-Spondin 1 / RSPO1 Protein SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"