Human R-Spondin 1 / RSPO1 (aa 1-146, His Tag) recombinant protein

Human R-Spondin 1 / RSPO1 (aa 1-146, His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

RSPO1 gene is a member of the R-spondin family. It encodes RSPO1 which is known as a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, RSPO1 induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. This protein is an activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. RSPO1 acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. It also acts as a ligand for frizzled FZD8 and LRP6.

R-spondin-1

RSPO; RP11-566C13.1; R-SPONDIN1; FLJ40906; R-SPONDIN; RSPO1; RSPONDIN; RP23-325M14.2; CRISTIN3

Q2MKA7

RSPO1

Human Cells

A DNA sequence encoding the amino acids (Met 1-Ala 146) of human RSPO1 (NP_001033722.1) was expressed, fused with a polyhistidine tag at the C-terminus.

Human

The secreted recombinant human RSPO1 (aa 1-146) comprises 137 amino acids and has a predicted molecular mass of 14 kDa. As a result of glycosylation, rhRSPO1 migrates as an approximately 23 kDa band in SDS-PAGE under reducing conditions.

> 98 % as determined by SDS-PAGE

Measured by its ability to bind recombinant mouse CD36 in a functional ELISA.