Mouse R-Spondin 1 / RSPO1 (His Tag) recombinant protein

Mouse R-Spondin 1 / RSPO1 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

RSPO1 gene is a member of the R-spondin family. It encodes RSPO1 which is known as a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, RSPO1 induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. This protein is an activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. RSPO1 acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. It also acts as a ligand for frizzled FZD8 and LRP6.

R-spondin homolog (Xenopus laevis)

R-SPONDIN; RSPO1; RSPONDIN; RP23-325M14.2; CRISTIN3; RSPO; RP11-566C13.1; R-SPONDIN1; FLJ40906

Q9Z132

Rspo1; mCG_17246

CHO Cells

A DNA sequence encoding the full length of mouse RSPO1 (NP_619624.2) (Met 1-Gln 265) was fused with a polyhistidine tag at the C-terminus.

Mouse

The secreted recombinant mouse RSPO1 comprises 256 amino acids with a predicted molecular mass of 28.5 kDa. As a result of glycosylation, the apparent molecular mass of the protein is approximately 44 kDa in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

Measured by its ability to induce activation of β-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED₅₀ for this effect is typically 50-200 ng/ml in the presence of 50 ng/ml recombinant mouse Wnt3a.