Mouse R-Spondin 1 / RSPO1 (His Tag) recombinant protein

Cat.#: 500914

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Product Information

  • Product Name
    Mouse R-Spondin 1 / RSPO1 (His Tag) recombinant protein
  • Documents
  • Description
    RSPO1 gene is a member of the R-spondin family. It encodes RSPO1 which is known as a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, RSPO1 induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. This protein is an activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. RSPO1 acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. It also acts as a ligand for frizzled FZD8 and LRP6.
  • Protein name
    R-spondin homolog (Xenopus laevis)
  • Protein short names
    R-SPONDIN; RSPO1; RSPONDIN; RP23-325M14.2; CRISTIN3; RSPO; RP11-566C13.1; R-SPONDIN1; FLJ40906
  • Uniprot ID
    Q9Z132
  • Gene Name
    Rspo1; mCG_17246
  • Source/Expression Host
    CHO Cells
  • Expression Plasmid/cDNA
    A DNA sequence encoding the full length of mouse RSPO1 (NP_619624.2) (Met 1-Gln 265) was fused with a polyhistidine tag at the C-terminus.
  • Protein Species
    Mouse
  • Molecular weight
    The secreted recombinant mouse RSPO1 comprises 256 amino acids with a predicted molecular mass of 28.5 kDa. As a result of glycosylation, the apparent molecular mass of the protein is approximately 44 kDa in SDS-PAGE under reducing conditions.
  • Purity
    > 95 % as determined by SDS-PAGE
  • Activity
    Measured by its ability to induce activation of β-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 50-200 ng/ml in the presence of 50 ng/ml recombinant mouse Wnt3a.
  • Validations

    Mouse R-Spondin 1 / RSPO1 Protein (His Tag) SDS-PAGE

    Mouse R-Spondin 1 / RSPO1 Protein (His Tag) SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"