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Product Name
Cynomolgus TNF-alpha/TNFA recombinant protein
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Description
Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.
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Protein name
Putative uncharacterized protein
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Protein short names
DADB-70P7.1; DIF; TNFSF1A; MGC151434; TNF; TNFSF2; TNF-A; TNFA; TNFALPHA; TNF-ALPHA
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Uniprot ID
P48094
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Gene Name
EGK_14705
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
A DNA sequence encoding the cynomolgus TNF [(Identical to the rhesus TNF (NP_001040614.1)] (Val77-Leu233) was expressed and purified with an initial Met.
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Protein Species
Cynomolgus
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Molecular weight
The recombinant cynomolgus TNF consists of 158 amino acids and has a calculated molecular mass of 17.4 kDa.
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Purity
> 90 % as determined by SDS-PAGE
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Activity
Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 50-300 pg/ml.
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Validations
Cynomolgus TNF-alpha / TNFA / TNFSF1A / Cachectin Protein SDS-PAGE
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"