Rat PD-L1/B7-H1/CD274 (Fc Tag) recombinant protein

Rat PD-L1/B7-H1/CD274 (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. PD-L1/B7-H1 is a member of the growing B7 family of immune molecules and this protein contains one V-like and one C-like Ig domain within the extracellular domain, and together with PD-L2, are two ligands for PD1 which belongs to the CD28/CTLA4 family expressed on activated lymphoid cells. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.

Protein Cd274

D4AE25

Cd274; RGD1566211_predicted; rCG_48457

Human Cells

A DNA sequence encoding the rat CD274 (NP_001178883.1)(Met1-Thr238) was expressed with the Fc region of human IgG1 at the C-terminus.

Rat

The recombinant rat CD274/Fc is a disulfide-linked homodimer. The reduced monomer comprises 459 amino acids and has a predicted molecular mass of 51.8 kDa. The apparent molecular mass of the protein is approximately 73.2 kDa in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
Immobilized rat CD274-Fc at 10 μg/ml (100 μl/well) can bind biotinylated rat PDCD1-Fc (Cat:501471), The EC₅₀ of biotinylated rat PDCD1-Fc (Cat:501471) is 0.14-0.34 μg/ml.