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Product Name
PD-L1/CD274 antibody
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Description
PD-L1/CD274 Rabbit Polyclonal antibody. Positive WB detected in mouse heart tissue, A549 cells, HeLa cells, HepG2 cells, human heart tissue, K-562 cells, mouse skeletal muscle tissue. Positive IP detected in mouse heart tissue. Positive IF detected in HEK-293 cells. Positive IHC detected in mouse heart tissue, human heart tissue, human stomach cancer tissue. Observed molecular weight by Western-blot: 50-56 kDa, 65-70 kDa
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Tested applications
ELISA, WB, IF, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PD-L1/CD274 recombinant protein (Accession Number: NM_014143). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:113628(PD-L1/CD274 Antibody) at dilution of 1:300
Immunohistochemistry of paraffin-embedded mouse heart tissue slide using Catalog No:113628(PD-L1/CD274 Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded mouse heart tissue slide using Catalog No:113628(PD-L1/CD274 Antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HEK-293 cells using Catalog No:113628(PD-L1/CD274 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
IP Result of anti-PD-L1/CD274 (IP:Catalog No:113628, 4ug; Detection:Catalog No:113628 1:1000) with mouse heart tissue lysate 4000ug.
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Background
PD-L1 (programmed cell death ligand 1, also known as CD274 or B7-H1) is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested a negative regulator of T and B cell, and play important role in mediating tolerance of lymphocytes to self-antigens. It also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD1-independent manner. PD-L1 is a 290 aa transmembrane protein with a calculated molecular weight of 33 kDa, the apparent molecular weight has been reported as 50 kDa or 65 kDa, suggesting probable glycosylation (PMID: 18760278; 16493058). Read more about this antibody on the blog: PD-L1 and Lung Cancer Research: Pathways to Promising Immunotherapies
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References
- Yang CY, Lin MW, Chang YL, Wu CT, Yang PC. Programmed cell death-ligand 1 expression in surgically resected stage I pulmonary adenocarcinoma and its correlation with driver mutations and clinical outcomes. European journal of cancer (Oxford, England : 1990). 50(7):1361-9. 2014.
- Han L, Liu F, Li R. Role of programmed death ligands in effective T-cell interactions in extranodal natural killer/T-cell lymphoma. Oncology letters. 8(4):1461-1469. 2014.
- Jiang L, Zhao Z, Jiang S. Immunological markers predict the prognosis of patients with squamous non-small cell lung cancer. Immunologic research. 62(3):316-24. 2015.
- Kao CJ, Wurz GT, Lin YC. Assessing the Effects of Concurrent versus Sequential Cisplatin/Radiotherapy on Immune Status in Lung Tumor-Bearing C57BL/6 Mice. Cancer immunology research. 3(7):741-50. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"