Mouse PD-L1/B7-H1/CD274 (His & Fc Tag) recombinant protein

Mouse PD-L1/B7-H1/CD274 (His & Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. PD-L1/B7-H1 is a member of the growing B7 family of immune molecules and this protein contains one V-like and one C-like Ig domain within the extracellular domain, and together with PD-L2, are two ligands for PD1 which belongs to the CD28/CTLA4 family expressed on activated lymphoid cells. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.

CD274 antigen

B7-H; MGC142296; CD274 A530045L16RIK; MGC142294; PDCD1LG1; PDL1; PDCD1L1; B7H1; PD-L1; CD274

Q9EP73

Cd274; mCG_9108

Human Cells

A DNA sequence encoding the extracellular domain (Met 1-Thr 238) of mouse PD-L1 (NP_068693.1) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus.

Mouse

The recombinant mouse PD-L1/Fc is a disulfide-linked homodimeric protein after proteolytic removal of the signal peptide. The reduced monomer consists of 468 amino acids and predicts a molecular mass of 52.8 kDa. As a result of glycosylation, the apparent molecular mass of rm PD-L1/Fc monomer is approximately 65-75 kDa in SDS-PAGE under reducing conditions.

> 97 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse PD-1 at 10 μg/ml (100 μl/well) can bind biotinylated mouse PD-L1 with a linear range of 3.2-400 ng/ml.