Cynomolgus PD-L1/B7-H1/CD274 (Fc Tag) recombinant protein

Cynomolgus PD-L1/B7-H1/CD274 (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. PD-L1/B7-H1 is a member of the growing B7 family of immune molecules and this protein contains one V-like and one C-like Ig domain within the extracellular domain, and together with PD-L2, are two ligands for PD1 which belongs to the CD28/CTLA4 family expressed on activated lymphoid cells. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.

Programmed cell death 1 ligand 1

B7-H; MGC142296; CD274 A530045L16RIK; MGC142294; PDCD1LG1; PDL1; PDCD1L1; B7H1; CD274; PD-L1

F6VEW6

CD274; EGK_07725

Human Cells

A DNA sequence encoding the cynomolgus CD274 (F6VEW6) (Met1-Thr239) was expressed with the Fc region of human IgG1 at the C-terminus.

Cynomolgus

The recombinant cynomolgus CD274 comprises 462 amino acids and has a calculated molecular mass of 52.3 KDa.The apparent molecular mass of the protein is approximately 62-68 KDa respectively in SDS-PAGE.

> 95 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
Immobilized human PD1-His (Cat:503606) at 10 μg/ml (100 μl/well) can bind Cynomolgus CD274-Fc (cat:500193), The EC₅₀ of Cynomolgus CD274-Fc (cat:500193) is 0.5-1.3 μg/ml.