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Product Name
Canine EGF/Epidermal Growth Factor (His Tag) recombinant protein
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Description
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6 (By similarity).
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Protein name
Pro-epidermal growth factor
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Protein short names
HOMG4; URG; AI790464; EGF
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Uniprot ID
Q9BEA0
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Gene Name
EGF
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
A DNA sequence encoding the mature form of canine EGF (Q9BEA0) (Asn 973-Arg 1024) was fused with a polyhistidine tag at the N-terminus.
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Protein Species
Canine
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Molecular weight
The recombinant canine EGF consists of 68 amino acids and has a calculated molecular mass of 8.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of canine EGF is approximately 12 kDa.
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Purity
> 98 % as determined by SDS-PAGE
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Activity
Measured in a cell proliferation assay using Balb 3T3 mouse embryonic fibroblasts. The EC50 for this effect is typically 0.1-0.5 ng/ml.
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Validations
Canine EGF / Epidermal Growth Facto Protein (His Tag) SDS-PAGE
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