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Product Name
Rat EGF/Epidermal Growth Factor recombinant protein
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Description
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6 (By similarity).
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Protein name
Pro-epidermal growth factor
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Protein short names
EGF; HOMG4; URG; AI790464
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Uniprot ID
P07522
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Gene Name
Egf
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
A DNA sequence encoding the rat EGF (NP_036974.1) (Asn974-Arg1026) was expressed and purified with an initial Met.
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Protein Species
Rat
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Molecular weight
The recombinant rat EGF consists of 54 amino acids and has a predicted molecular mass of 6.3 kDa. The apparent molecular mass of the recombinant protein is approximately 6-9 kDa in SDS-PAGE under reducing conditions.
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Purity
> 95 % as determined by SDS-PAGE
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Activity
Measured in a cell proliferation assay using BALB/c 3T3 mouse fibroblasts.
The ED50 for this effect is typically 0.01-0.05 ng/ml. -
Validations
Rat EGF / Epidermal Growth Factor Protein SDS-PAGE
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