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  • Anti-EGF/Epidermal Growth Factor antibody

Anti-EGF/Epidermal Growth Factor antibody

Cat.#: 101047

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Product Information

  • Product Name
    Anti-EGF/Epidermal Growth Factor antibody
  • Documents
  • Description
    Rabbit polyclonal to EGF/Epidermal Growth Factor
  • Tested applications
    ELISA, IHC-P
  • Species reactivity
    Rat EGF / Epidermal Growth Factor
  • Alternative names
    URG antibody; HOMG4 antibody; AI790464 antibody; AI790464 antibody; Egf antibody; EGF antibody; HOMG4 antibody; URG antibody
  • Immunogen
  • Isotype
    Rabbit IgG
  • Preparation
    Produced in rabbits immunized with purified, recombinant Rat EGF / Epidermal Growth Factor (rh EGF / Epidermal Growth Factor; NP_036974.1; Asn974-Arg1026). EGF / Epidermal Growth Factor specific IgG was purified by Rat EGF / Epidermal Growth Factor affinity chromatography.
  • Clonality
    Polyclonal
  • Formulation
    0.2 μm filtered solution in PBS
  • Storage instructions
    This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
    Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
  • Applications

    ELISA:0.1-0.2 μg/mL

    This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Rat EGF / Epidermal Growth Factor. The detection limit for Rat EGF / Epidermal Growth Factor is < 0.039 ng/well.

    IHC-P: 0.1-2 μg/ml

  • Validations

    EGF / Epidermal Growth Factor Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry

    EGF / Epidermal Growth Factor Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry

  • Background
    EGF is the founding member of the EGF-family of proteins. Members of this protein family have highly similar structural and functional characteristics. EGF contains 9 EGF-like domains and 9 LDL-receptor class B repeats. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bonds. As a low-molecular-weight polypeptide, EGF was first purified from the mouse submandibular gland, but since then it was found in many human tissues including submandibular gland, parotid gland. It can also be found in human platelets, macrophages, urine, saliva, milk, and plasma. EGF is a growth factor that stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. It results in cellular proliferation, differentiation, and survival. Salivary EGF, which seems also regulated by dietary inorganic iodine, also plays an important physiological role in the maintenance of oro-esophageal and gastric tissue integrity. EGF acts by binding with high affinity to epidermal growth factor receptor on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. The tyrosine kinase activity, in turn, initiates a signal transduction cascade that results in a variety of biochemical changes within the cell - a rise in intracellular calcium levels, increased glycolysis and protein synthesis, and increases in the expression of certain genes including the gene for EGFR - that ultimately lead to DNA synthesis and cell proliferation.
  • References
    • Chen JX, et al. (2011) Involvement of c-Src/STAT3 signal in EGF-induced proliferation of rat spermatogonial stem cells. Mol Cell Biochem. 358(1-2):67-73.
    • Guo Y, et al. (2012) Correlations among ERCC1, XPB, UBE2I, EGF, TAL2 and ILF3 revealed by gene signatures of histological subtypes of patients with epithelial ovarian cancer. Oncol Rep. 27(1):286-92.
    • Kim S, et al. (2012) Smad7 acts as a negative regulator of the epidermal growth factor (EGF) signaling pathway in breast cancer cells. Cancer Lett. 314(2):147-54.
    • Chatterton RT Jr, et al. (2010) Breast ductal lavage for assessment of breast cancer biomarkers. Horm Cancer. 1(4):197-204.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"