Mouse EGF/Epidermal Growth Factor recombinant protein

Mouse EGF/Epidermal Growth Factor recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

EGF is the founding member of the EGF-family of proteins. Members of this protein family have highly similar structural and functional characteristics. EGF contains 9 EGF-like domains and 9 LDL-receptor class B repeats. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bonds. As a low-molecular-weight polypeptide, EGF was first purified from the mouse submandibular gland, but since then it was found in many human tissues including submandibular gland, parotid gland. It can also be found in human platelets, macrophages, urine, saliva, milk, and plasma. EGF is a growth factor that stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. It results in cellular proliferation, differentiation, and survival. Salivary EGF, which seems also regulated by dietary inorganic iodine, also plays an important physiological role in the maintenance of oro-esophageal and gastric tissue integrity. EGF acts by binding with high affinity to epidermal growth factor receptor on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. The tyrosine kinase activity, in turn, initiates a signal transduction cascade that results in a variety of biochemical changes within the cell - a rise in intracellular calcium levels, increased glycolysis and protein synthesis, and increases in the expression of certain genes including the gene for EGFR - that ultimately lead to DNA synthesis and cell proliferation.

Pro-epidermal growth factor

EGF; AI790464; URG; HOMG4

P01132

Egf

Yeast

A DNA sequence encoding the mouse EGF (P01132) (Asn977-Arg1029) was expressed and purified.

Mouse

The recombinant mouse EGF consists of 53 amino acids and predicts a molecular mass of 6 KDa. It migrates as an approximately 6 KDa band in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells.
The ED₅₀ for this effect is typically 20-100 pg/mL.