PKM antibody

Cat.#: 113858

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Product Information

  • Product Name
    PKM antibody
  • Documents
  • Description
    PKM Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, COLO 320 cells, HEK-293 cells, MCF-7 cells, SMMC-7721 cells. Observed molecular weight by Western-blot: 55 kDa
  • Tested applications
    ELISA, WB
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    CTHBP antibody; OIP 3 antibody; OIP3 antibody; Opa interacting protein 3 antibody; p58 antibody; PK2 antibody; PK3 antibody; PKM antibody; PKM1 antibody; PKM2 antibody; PKM2 pyruvate kinase antibody; muscle antibody; Pyruvate kinase 2/3 antibody; Pyruvate kinase isozymes M1/M2 antibody; Pyruvate kinase muscle isozyme antibody; TCB antibody; THBP1 antibody; Tumor M2 PK antibody
  • Immunogen
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of PKM recombinant protein (Accession Number: NM_002654). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113858(PKM Antibody) at dilution of 1:1000

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113858(PKM Antibody) at dilution of 1:1000

  • Background
    PKM, also named as OIP3, PK2, PK3, PK-M1/2, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. The activity of the M2 isoform (but not the M1 isoform) can be inhibited by tyrosine kinase signalling in tumourcells.The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2.(PMID:18337823). PKM, Pyruvate kinase isozymes M1/M2, has 2 isoforms . This antibody can recognize both PK-M1 and PK-M2.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"