Anti-Phospho-PKM2 (Ser37) Rabbit antibody

Cat.#: 166999

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Product Information

  • Product Name
    Anti-Phospho-PKM2 (Ser37) Rabbit antibody
  • Documents
  • Description
    Phospho-PKM2 (Ser37) Rabbit polyclonal antibody
  • Tested applications
    WB, ICC/IF
  • Species reactivity
    Human
  • Isotype
    IgG
  • Preparation
    Antigen: Peptide sequence around phosphorylation site of serine 37(I-D-S(p)-P-P) derived from Human PKM2.
  • Clonality
    Polyclonal
  • Formulation
    Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB:1/500-1/1000ICC:1/100-1/200

  • Validations

    Western blot analysis of in vitro kinase assays carried out with puried active ERK2, wild-type (WT) PKM2 and PKM2 S37A mutant using PKM2(phospho-Ser37)Antibody .

    Western blot analysis of in vitro kinase assays carried out with puried active ERK2, wild-type (WT) PKM2 and PKM2 S37A mutant using PKM2(phospho-Ser37)Antibody .

    Western blot detection of PKM2(Phospho-Ser37)  in A549 cells untreated or treated with Alkaline phosphatase using PKM2(Phospho-Ser37) Rabbit pAb (dilution 1:500, upper) or PKM2 Mouse mAb (200667-1A7,dilution 1:1000, lower)).Predicted band size:60kDa.Observed band size:60kDa.

    Western blot detection of PKM2(Phospho-Ser37) in A549 cells untreated or treated with Alkaline phosphatase using PKM2(Phospho-Ser37) Rabbit pAb (dilution 1:500, upper) or PKM2 Mouse mAb (200667-1A7,dilution 1:1000, lower)).Predicted band size:60kDa.Observed band size:60kDa.

    Immunofluorescence staining of methanol-fixed MEF cells using PKM2 (phospho-Ser37) Antibody .

    Immunofluorescence staining of methanol-fixed MEF cells using PKM2 (phospho-Ser37) Antibody .

  • Background
    Swiss-Prot Acc.P14618.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"