-
Product Name
Anti-Phospho-PKM2 (Ser37) Rabbit antibody
- Documents
-
Description
Phospho-PKM2 (Ser37) Rabbit polyclonal antibody
-
Tested applications
WB, ICC/IF
-
Species reactivity
Human
-
Isotype
IgG
-
Preparation
Antigen: Peptide sequence around phosphorylation site of serine 37(I-D-S(p)-P-P) derived from Human PKM2.
-
Clonality
Polyclonal
-
Formulation
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
-
Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
-
Applications
WB:1/500-1/1000ICC:1/100-1/200
-
Validations
Western blot analysis of in vitro kinase assays carried out with puried active ERK2, wild-type (WT) PKM2 and PKM2 S37A mutant using PKM2(phospho-Ser37)Antibody .
Western blot detection of PKM2(Phospho-Ser37) in A549 cells untreated or treated with Alkaline phosphatase using PKM2(Phospho-Ser37) Rabbit pAb (dilution 1:500, upper) or PKM2 Mouse mAb (200667-1A7,dilution 1:1000, lower)).Predicted band size:60kDa.Observed band size:60kDa.
Immunofluorescence staining of methanol-fixed MEF cells using PKM2 (phospho-Ser37) Antibody .
-
Background
Swiss-Prot Acc.P14618.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"