PKM antibody

PKM antibody

• DataSheet
• Material Safety Data Sheets (MSDS)

PKM Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue. Positive WB detected in multi-cells/tissue, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, MCF7 cells, NIH/3T3 cells, Raji cells, SH-SY5Y cells, SKOV-3 cells. Observed molecular weight by Western-blot: 58 kDa

ELISA, IHC, WB

Human, Mouse; other species not tested.

CTHBP antibody; OIP 3 antibody; OIP3 antibody; Opa interacting protein 3 antibody; p58 antibody; PK2 antibody; PK3 antibody; PKM antibody; PKM1 antibody; PKM2 antibody; PKM2 pyruvate kinase antibody; muscle antibody; Pyruvate kinase 2/3 antibody; Pyruvate kinase isozymes M1/M2 antibody; Pyruvate kinase muscle isozyme antibody; TCB antibody; THBP1 antibody; Tumor M2 PK antibody

Rabbit IgG

This antibody was obtained by immunization of PKM recombinant protein (Accession Number: NM_002654). Purification method: Antigen affinity purified.

Polyclonal

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Store at -20℃. DO NOT ALIQUOT

PKM, also named as OIP3, PK2, PK3, PK-M1/2, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. The activity of the M2 isoform (but not the M1 isoform) can be inhibited by tyrosine kinase signalling in tumourcells.The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2.(PMID:18337823). PKM, Pyruvate kinase isozymes M1/M2, has 2 isoforms . This antibody can recognize both PK-M1 and PK-M2.

• Yu Y, Pan X, Ding Y. An iTRAQ based quantitative proteomic strategy to explore novel secreted proteins in metastatic hepatocellular carcinoma cell lines. The Analyst. 138(16):4505-11. 2013.