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Product Name
PAIP1 antibody
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Description
PAIP1 Mouse Monoclonal antibody. Positive WB detected in HeLa cells, human brain tissue, human placenta tissue. Positive IF detected in HeLa cells. Positive IHC detected in human testis tissue, human cervical cancer tissue. Observed molecular weight by Western-blot: 42+60+70+110 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
PABP interacting protein 1 antibody; PAIP 1 antibody; PAIP1 antibody
- Immunogen
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Isotype
Mouse IgG2b
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Preparation
This antibody was obtained by immunization of PAIP1 recombinant protein (Accession Number: NM_182789). Purification method: Caprylic Acid/Ammonium Sulfate Precipitation.
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Clonality
Monoclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107450(PAIP1 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human testis slide using Catalog No:107450(PAIP1 Antibody) at dilution of 1:50
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:107450(PAIP1 Antibody) at dilution of 1:200 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
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Background
In initiation of translation in eukaryotes, binding of the small ribosomal subunit to mRNA requires recognition of the 5-prime cap structure by the cap-binding complex eIF4F. eIF4F consists of eIF4E, eIF4A, and eIF4G. Translation initiation is further regulated by the mRNA 3-prime poly(A) tail and the poly(A)-binding protein (PABC1). PAIP1 interacts with PABC1 and some eIF4 complexes [PMID:9548260]. It is a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs, and associates with EIF4A and PABPC1 may potentiate contacts between mRNA termini [PMID:11051545].
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