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PAIP1 antibody

Cat.#: 113494

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Product Information

  • Product Name
    PAIP1 antibody
  • Documents
  • Description
    PAIP1 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, BxPC-3 cells, COS-7 cells. Positive IF detected in MCF-7 cells. Observed molecular weight by Western-blot: 60 kDa,51 kDa,40 kDa
  • Tested applications
    ELISA, WB, IF
  • Species reactivity
    Human,Mouse,Rat, Monkey; other species not tested.
  • Alternative names
    PABP interacting protein 1 antibody; PAIP 1 antibody; PAIP1 antibody
  • Immunogen
    Human PAIP1 Recombinant protein (GST tag)
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of PAIP1 recombinant protein (Accession Number: NM_182789). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IF: 1:20-1:200

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113494(PAIP1 antibody) at dilution of 1:1000

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113494(PAIP1 antibody) at dilution of 1:1000

    Immunofluorescent analysis of MCF-7 cells, using PAIP1 antibody Catalog No:113494 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

    Immunofluorescent analysis of MCF-7 cells, using PAIP1 antibody Catalog No:113494 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Background
    In initiation of translation in eukaryotes, binding of the small ribosomal subunit to mRNA requires recognition of the 5-prime cap structure by the cap-binding complex eIF4F. eIF4F consists of eIF4E, eIF4A, and eIF4G. Translation initiation is further regulated by the mRNA 3-prime poly(A) tail and the poly(A)-binding protein (PABC1). PAIP1 interacts with PABC1 and some eIF4 complexes [PMID:9548260]. It is a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs, and associates with EIF4A and PABPC1 may potentiate contacts between mRNA termini [PMID:11051545].
  • References
    • Mead EJ, Masterton RJ, Feary M. Biological insights into the expression of translation initiation factors from recombinant CHOK1SV cell lines and their relationship to enhanced productivity. The Biochemical journal. 472(3):261-73. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"