Mouse Junctional Adhesion Molecule B (His Tag) recombinant protein

Mouse Junctional Adhesion Molecule B (His Tag) recombinant protein

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Junctional adhesion molecule B (JAM-B), also known as Junctional adhesion molecule 2 (JAM2), Vascular endothelial junction-associated molecule (VE-JAM), and CD322, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily. It is prominently expressed on high endothelial venules. expression to be restricted to the high endothelial venule of tonsil and lymph nodes. The localization to the endothelium of arterioles in and around inflammatory and tumor foci. JAM-B can function as an adhesive ligand for the T cell line J45 and can interact with GM-CSF/IL-4-derived peripheral blood dendritic cells, circulating CD56(+) NK cells, circulating CD56(+)CD3(+) NK/T cells, and circulating CD56(+)CD3(+)CD8(+) cytolytic T cells. JAM-2 is expressed on high endothelial venules (HEVs) in human tonsil and on a subset of human leukocytes, suggesting that JAM-2 plays a central role in the regulation of transendothelial migration. It binds to very late activation antigen (VLA)-4, a leucocyte integrin that contributes to rolling and firm adhesion of lymphocytes to endothelial cells through binding to vascular cell adhesion molecule (VCAM)-1. JAM-B appears to contribute to leucocyte extravasation by facilitating not only transmigration but also rolling and adhesion. JAM-B acts as an adhesive ligand for interacting with a variety of immune cell types and may play a role in lymphocyte homing to secondary lymphoid organs.

Junctional adhesion molecule B

2410167M24RIK; RP23-253E17.2; VEJAM; VE-JAM; AU016127; C21ORF43; 2410030G21RIK; JAM2; PRO245; JAM-2; JAM-B; UNQ219/PRO245; CD322; JCAM2; JAMB; JAM-IT/VE-JAM; 1110002N23RIK

Q9JI59

Jam2; Vejam

Human Cells

A DNA sequence encoding the extracellular domain of mouse JAM2 (NP_076333.3) (Met 1-Asn 236) was expressed, with a polyhistidine tag at the C-terminus.

Mouse

The recombinant mouse JAM2 consists of 219 amino acids and has a predicted molecular mass of 24.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm JAM2 is approximately 37 kDa due to glycosylation.

> 97 % as determined by SDS-PAGE

Measured by its ability of the immobilized protein to support the adhesion of Jurkat human leukemic T cells. When 8 x 10E4 cells/well are added to JAM2 coated plates (0.2 μg/ml and 100 μl/well), approximately 35-60% will adhere specifically after 60 minutes at 37℃.