Mouse HGF / Hepatocyte Growth Factor recombinant protein

Mouse HGF / Hepatocyte Growth Factor recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Hepatocyte growth factor, also known as HGF, contains 4 kringle domains, 1 PAN domain and 1 peptidase S1 domain. It belongs to the peptidase S1 family, plasminogen subfamily. Hepatocyte growth factor is secreted by mesenchymal cellsas a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor, and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. HGF is a potent mitogen for mature parenchymal hepatocyte cells, seems to be an hepatotrophic factor, and acts as growth factor for a broad spectrum of tissues and cell types. HGF has no detectable protease activity. Defects in hepatocyte growth factor are the cause of deafness autosomal recessive type 39. A form of profound prelingual sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.

Hepatocyte growth factor

C230052L06RIK; SF/HGF; F-TCF; SF; NK1; HGF/SF; NK2; HGFB; HGF; DFNB39; HPTA

Q08048

Hgf

Human Cells

A DNA sequence encoding the mouse HGF (Q08048-1) (Met1-Leu728) was expressed and purified.

Mouse

The recombinant mouse HGF comprises 696 amino acids and has a predicted molecular mass of 79.3 kDa. The apparent molecular mass of the protein is approximately 93 kDa in SDS-PAGE under reducing conditions due to glycosylation.

> 95 % as determined by SDS-PAGE

1. Immobilized mouse HGF at 10 μg/ml (100 μl/well) can bind mouse MET-Fc (Cat:503341), EC₅₀ of mouse MET-Fc (Cat:503341) is 0.05-0.12 μg/ml.
2. Measured by its ability to inhibit TGFß1 activity on Mv-1-lu mink lung epithelial cells. The ED₅₀ for this effect is typically 1-4 ng/ml.