Anti-HGF / Hepatocyte Growth Factor antibody

Anti-HGF / Hepatocyte Growth Factor antibody

• DataSheet
• Material Safety Data Sheets (MSDS)

Mouse monoclonal to HGF / Hepatocyte Growth Factor

B/N

Human HGF / Hepatocyte Growth Factor
No cross-reactivity with Mouse HGF (503003) and Rat HGF (504728) in ELISA assay

sf antibody; HGF antibody; SF antibody; HGFB antibody; HPTA antibody; F-TCF antibody; DFNB39 antibody; NK1 antibody; NK2 antibody; HGF/SF antibody; SF/HGF antibody; C230052L06Rik antibody; SF antibody; HGF antibody; C230052L06Rik antibody; DFNB39 antibody; F-TCF antibody; HGF/SF antibody; HGFB antibody; HPTA antibody; NK1 antibody; NK2 antibody; SF/HGF antibody

Mouse (varialbe region) / Human (kappa / IgG1 constant)

It is a chimeric monoclonal antibody combining the constant domains of the human IgG1 molecule with mouse variable regions. The variable region was obtained from a mouse immunized with purified, recombinant Human HGF / Hepatocyte Growth Factor. The antibody was produced using recombinant antibody technology.

Monoclonal

0.2 μm filtered solution in PBS

This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Block - This HGF Neutralizing Antibody can block the binding of recombinant human HGF Protein to its recombinant receptor HGFR.
Neutralization - The neutralization activity of HGF Neutralizing Antibody is Measured by its ability to neutralize autocrine HGF-induced proliferation in the U87 MG cell line. Proliferation of U87 MG cells elicited by autocrine HGF was inhibited by increasing concentrations of Human HGF Monoclonal Antibody. The IC50 is typically 1-4 μg/mL.

Hepatocyte growth factor, also known as HGF, contains 4 kringle domains, 1 PAN domain and 1 peptidase S1 domain. It belongs to the peptidase S1 family, plasminogen subfamily. Hepatocyte growth factor is secreted by mesenchymal cellsas a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor, and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. HGF is a potent mitogen for mature parenchymal hepatocyte cells, seems to be an hepatotrophic factor, and acts as growth factor for a broad spectrum of tissues and cell types. HGF has no detectable protease activity. Defects in hepatocyte growth factor are the cause of deafness autosomal recessive type 39. A form of profound prelingual sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.

• Naldini L, et al. (1991) Scatter factor and hepatocyte growth factor are indistinguishable ligands for the MET receptor. EMBO J. 10(10):2867-78.
• Comoglio, et al. (1993) Structure, biosynthesis and biochemical properties of the HGF receptor in normal and malignant cells. 65:131-65.
• Hahn W, et al. (2011) Enhanced cardioprotective effects by coexpression of two isoforms of hepatocyte growth factor from naked plasmid DNA in a rat ischemic heart disease model. The Journal of Gene Medicine. 13(10):549-55.
• Bottaro DP, et al. (1991) Identification of the hepatocyte growth factor receptor as the c-met proto-oncogene product. Science. 251(4995):802-4.