Mouse EphB2/Eph Receptor B2 (Fc Tag) recombinant protein

Mouse EphB2/Eph Receptor B2 (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Ephrin type-B receptor 2, also known as EphB2, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. EphB2 receptor tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.

ERK; CAPB; DRT; QEK5; CEK5; EPHT3; NUK; SEK3; ETECK; HEK5; TYRO5; PCBC; EPHB2; MGC87492; RP23-426N4.7; EK5; PRKM5

P54763

Human Cells

A DNA sequence encoding the extracellular domain of mouse EPHB2 (NP_034272.1) (Met1-Lys540) was expressed with the Fc region of human IgG1 at the C-terminus.

Mouse

The recombinant mouse EPHB2 comprises 760 amino acids and has a predicted molecular mass of 84.6 kDa. The apparent molecular mass of the protein is approximately 96.6 kDa in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

1. Measured by its binding ability in a functional ELISA.
2. Immobilized mouse EFNB2-His (Cat:500099) at 10 μg/mL (100 μL/well) can bind mouse EPHB2-Fc. The EC₅₀ of mouse EPHB2-Fc is 13.2-30.8ng/mL.