Human EphB2/Eph Receptor B2 (aa 570-987, His & GST Tag) recombinant protein

Human EphB2/Eph Receptor B2 (aa 570-987, His & GST Tag) recombinant protein

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Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Functions in axon guidance during development. Involved in the guidance of commissural axons, that form a major interhemispheric connection between the 2 temporal lobes of the cerebral cortex. Also involved in guidance of contralateral inner ear efferent growth cones at the midline and of retinal ganglion cell axons to the optic disk. In addition to axon guidance, also regulates dendritic spines development and maturation and stimulates the formation of excitatory synapses. Upon activation by EFNB1, abolishes the ARHGEF15-mediated negative regulation on excitatory synapse formation. Controls other aspects of development including angiogenesis, palate development and in inner ear development through regulation of endolymph production. Forward and reverse signaling through the EFNB2/EPHB2 complex regulate movement and adhesion of cells that tubularize the urethra and septate the cloaca. May function as a tumor suppressor.

Ephrin type-B receptor 2

MGC87492; EK5; RP23-426N4.7; PRKM5; HEK5; EPHB2; PCBC; TYRO5; EPHT3; CEK5; QEK5; ETECK; SEK3; NUK; CAPB; DRT; ERK

P29323

EPHB2; DRT; EPHT3; EPTH3; ERK; HEK5; TYRO5

Baculovirus-Insect Cells

A DNA sequence encoding the human EPHB2 (P29323-3) (Gly570-Val987) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Human

The recombinant human EPHB2 /GST chimera consists of 655 amino acids and has a calculated molecular mass of 75.2 kDa. The recombinant protein migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.

> 90 % as determined by SDS-PAGE

The specific activity was determined to be 120 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate.