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Product Name
Human EphB2/Eph Receptor B2 (His & Fc Tag) recombinant protein
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Description
Ephrin type-B receptor 2, also known as EphB2, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. EphB2 receptor tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.
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Protein name
Ephrin type-B receptor 2
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Protein short names
CAPB; DRT; ERK; SEK3; ETECK; NUK; EPHT3; QEK5; CEK5; PCBC; EPHB2; TYRO5; HEK5; RP23-426N4.7; EK5; PRKM5; MGC87492
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Uniprot ID
Q6NVW1
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Gene Name
EPHB2; DRT; EPHT3; EPTH3; ERK; HEK5; TYRO5
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the extracellular domain (Met 1-Leu 543) of human EphB2 (NP_059145.2) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
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Protein Species
Human
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Molecular weight
The recombinant human EphB2/Fc is a disulfide-linked homodimer. The reduced monomer consists of 773 amino acids and has a predicted molecular mass of 86 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh EphB2/Fc monomer is approximately 90-100 kDa due to glycosylation.
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Purity
> 90 % as determined by SDS-PAGE
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Activity
Measured by its ability to bind recombinant human EphrinB2 in a functional ELISA.
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Validations
Human EphB2 Protein (His & Fc Tag) SDS-PAGE
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