Human EphB2/Eph Receptor B2 recombinant protein

Human EphB2/Eph Receptor B2 recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Ephrin type-B receptor 2, also known as EphB2, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. EphB2 receptor tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.

Ephrin type-B receptor 2

CAPB; DRT; ERK; EPHT3; QEK5; CEK5; SEK3; ETECK; NUK; HEK5; PCBC; EPHB2; TYRO5; MGC87492; RP23-426N4.7; PRKM5; EK5

Q6NVW1

EPHB2; DRT; EPHT3; EPTH3; ERK; HEK5; TYRO5

Human Cells

A DNA sequence encoding the human EPHB2 (NP_059145.2) (Met 1-Leu 543) was expressed with six amino acids (ENLYFQ ) at the C-terminus.

Human

The recombinant human EPHB2 consists of 532 amino acids and predicts a molecular mass of 59 KDa. It migrates as an approximately 66 KDa band in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

Immobilized human EPHB2 at 10 μg/ml (100 μl/well) can bind human EFNB2-Fch (Cat:502066), The EC₅₀ of human EFNB2-Fch (Cat:502066) is 18.2-42.7 ng/ml.