Mouse CD64/FCGR1 (His Tag) recombinant protein

Mouse CD64/FCGR1 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

High affinity immunoglobulin gamma Fc receptor I, also known as FCGR1 and CD64, is an integral membrane glycoprotein and a member of the immunoglobulin superfamily. CD64 is a high affinity receptor for the Fc region of IgG gamma and functions in both innate and adaptive immune responses. Receptors that recognize the Fc portion of IgG function in the regulation of immune response and are divided into three classes designated CD64, CD32, and CD16. CD64 is structurally composed of a signal peptide that allows its transport to the surface of a cell, three extracellular immunoglobulin domains of the C2-type that it uses to bind antibody, a hydrophobic transmembrane domain, and a short cytoplasmic tail. CD64 is constitutively found on only macrophages and monocytes, but treatment of polymorphonuclear leukocytes with cytokines like IFN? and G-CSF can induce CD64 expression on these cells. The inactivation of the mouse CD64 resulted in a wide range of defects in antibody Fc-dependent functions. Mouse CD64 is an early participant in Fc-dependent cell activation and in the development of immune responses.

High affinity immunoglobulin gamma Fc receptor I

FCGR1C; CD64; IGFR1; FCGAMMARI; IGFRB; AV092959; FCGR1B; AI323638; RP11-439A17.1; FCRI; IGFRC; FCGR1A; IGGHAFC; FLJ18345; FCGR1

P26151

Fcgr1; Fcg1

Human Cells

A DNA sequence encoding the extracellular domain (Met 1-Pro 297) of mouse FCGR1 (NP_034316.1) precursor was fused with a polyhistidine tag at the C-terminus.

Mouse

The recombinant mouse FCGR1 consists of 284 amino acids after removal of the signal peptide and has a predicted molecular mass of 32 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm FCGR1 is approximately 45-50 kDa due to glycosylation.

> 97 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
Immobilized mouse CD64-His at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1, The EC₅₀ of biotinylated human IgG1 is 0.07-0.17 μg/ml.