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Product Name
Mouse CD64/FCGR1 (His & AVI Tag) recombinant protein
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Description
High affinity immunoglobulin gamma Fc receptor I, also known as FCGR1 and CD64, is an integral membrane glycoprotein and a member of the immunoglobulin superfamily. CD64 is a high affinity receptor for the Fc region of IgG gamma and functions in both innate and adaptive immune responses. Receptors that recognize the Fc portion of IgG function in the regulation of immune response and are divided into three classes designated CD64, CD32, and CD16. CD64 is structurally composed of a signal peptide that allows its transport to the surface of a cell, three extracellular immunoglobulin domains of the C2-type that it uses to bind antibody, a hydrophobic transmembrane domain, and a short cytoplasmic tail. CD64 is constitutively found on only macrophages and monocytes, but treatment of polymorphonuclear leukocytes with cytokines like IFN? and G-CSF can induce CD64 expression on these cells. The inactivation of the mouse CD64 resulted in a wide range of defects in antibody Fc-dependent functions. Mouse CD64 is an early participant in Fc-dependent cell activation and in the development of immune responses.
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Protein name
High affinity immunoglobulin gamma Fc receptor I
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Protein short names
FCGR1A; IGGHAFC; IGFRC; FLJ18345; FCGR1; RP11-439A17.1; FCRI; AI323638; IGFRB; AV092959; FCGR1B; FCGR1C; IGFR1; CD64; FCGAMMARI
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Uniprot ID
P26151
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Gene Name
Fcgr1; Fcg1
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the extracellular domain (Met 1-Pro 297) of mouse FCGR1 (NP_034316.1) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
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Protein Species
Mouse
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Molecular weight
The recombinant mouse FCGR1 consists of 308 amino acids after removal of the signal peptide and has a predicted molecular mass of 34.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm FCGR1 is approximately 50-55 kDa due to glycosylation.
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Purity
> 90 % as determined by SDS-PAGE
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Activity
1. Measured by its ability to bind mouse APCS in a functional ELISA.
2. Labeling ratio of biotin to protein: 1.1 -
Validations
Mouse CD64 / FCGR1 Protein (His & AVI Tag), Biotinylated SDS-PAGE
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