Mouse CD155/PVR (His & Fc Tag) recombinant protein

Mouse CD155/PVR (His & Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

CD155, commonly known as PVR (poliovirus receptor) and Necl-5 (nectin-like molecule-5), is a type I transmembrane single-span glycoprotein, and belongs to the nectins and nectin-like (Necl) subfamily. CD155 was originally identified based on its ability to mediate the cell attachment and entry of poliovirus (PV), an etiologic agent of the central nervous system disease poliomyelitis. The normal cellular function is in the establishment of intercellular adherens junctions between epithelial cells. CD155 may assist in an efficient humoral immune response generated within the intestinal immune system. It has been demonstrated that CD155 can be recognized and bond by DNAM-1 and CD96 which promote the adhension, migration and NK-cell killing, and thus efficiently prime cell-mediated tumor-specific immunity.

Nectin-2

HVED; TAA1; PVS; NECL5; NECL-5; D7ERTD458E; 3830421F03RIK; CD155; ME4; TAGE4; PVR

Q8K094

Pvr; D7Ertd458e; Tage4; mCG_4885

Human Cells

A DNA sequence encoding the mouse CD155 (NP_081790.1) precursor (Met 1-Arg 345) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Mouse

The secreted recombinant mouse CD155/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer comprises 566 amino acids with a predicted molecular mass of 63 kDa. As a result of glycosylation, it migrates as an approximately 95-105 kDa band in SDS-PAGE under reducing conditions.

> 90 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA. Immobilized mouse DNAM1 at 2 μg/ml (100 μl/well) can bind mouse CD155 with a linear ranger of 6.4-160 ng/ml.