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Product Name
Anti-CD155/PVR antibody
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Description
Mouse monoclonal to CD155/PVR
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Tested applications
ELISA, WB
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Species reactivity
Rhesus PVR / CD155
No cross-reactivity in ELISA with Human cell lysate (293 cell line) -
Alternative names
PVS antibody; HVED antibody; CD155 antibody; NECL5 antibody; TAGE4 antibody; Necl-5 antibody; PVS antibody; mE4 antibody; HVED antibody; Taa1 antibody; CD155 antibody; Tage4 antibody; necl-5 antibody; D7Ertd458e antibody; 3830421F03Rik antibody; 3830421F03Rik antibody; CD155 antibody; CD155 antibody; D7Ertd458e antibody; HVED antibody; HVED antibody; mE4 antibody; NECL5 antibody; necl-5 antibody; Necl-5 antibody; Pvr antibody; PVR antibody; PVS antibody; PVS antibody; Taa1 antibody; TAGE4 antibody; Tage4 antibody
- Immunogen
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Isotype
Mouse IgG1
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Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Rhesus PVR / CD155 (NP_001036851.1; Met 1-Asn 343). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 10-20 μg/ml
ELISA: 0.5-1 μg/mL
This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Rhesus PVR. The detection limit for Rhesus PVR is approximately 0.31 ng/well.
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Validations
CD155 / PVR Antibody, Mouse MAb, Western blot
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Background
CD155, commonly known as PVR (poliovirus receptor) and Necl-5 (nectin-like molecule-5), is a type I transmembrane single-span glycoprotein, and belongs to the nectins and nectin-like (Necl) subfamily. CD155 was originally identified based on its ability to mediate the cell attachment and entry of poliovirus (PV), an etiologic agent of the central nervous system disease poliomyelitis. The normal cellular function is in the establishment of intercellular adherens junctions between epithelial cells. CD155 may assist in an efficient humoral immune response generated within the intestinal immune system. It has been demonstrated that CD155 can be recognized and bond by DNAM-1 and CD96 which promote the adhension, migration and NK-cell killing, and thus efficiently prime cell-mediated tumor-specific immunity.
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References
- Freistadt MS, et al. (2000) Hematopoietic cells from CD155-transgenic mice express CD155 and support poliovirus replication ex vivo. Microb Pathog. 29(4): 203-12.
- Sato T, et al. (2004) Involvement of heterophilic trans-interaction of Necl-5/Tage4/PVR/CD155 with nectin-3 in formation of nectin- and cadherin-based adherens junctions. Genes Cells. 9(9): 791-9.
- Kakunaga S, et al. (2004) Enhancement of serum- and platelet-derived growth factor-induced cell proliferation by Necl-5/Tage4/poliovirus receptor/CD155 through the Ras-Raf-MEK-ERK signaling. J Biol Chem. 279(35): 36419-25.
- Sato T, et al. (2005) Common signaling pathway is used by the trans-interaction of Necl-5/Tage4/PVR/CD155 and nectin, and of nectin and nectin during the formation of cell-cell adhesion. Cancer Sci. 96(9): 578-89.
- Minami Y, et al. (2007) Involvement of up-regulated Necl-5/Tage4/PVR/CD155 in the loss of contact inhibition in transformed NIH3T3 cells. Biochem Biophys Res Commun. 352(4): 856-60.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"