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Product Name
Human p38 alpha/MAPK14 (His Tag) recombinant protein
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Description
MAPK14 contains 1 protein kinase domain and belongs to the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. MAPK14 can be detected in brain, heart, placenta, pancreas and skeletal muscle and it is expressed to a lesser extent in lung, liver and kidney. MAPK14 is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with MAPK14. The substrates of p38 alpha include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of p38 alpha in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. In respond to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide, MAPK14 phosphorylates a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. MAPK14 plays a critical role in the production of some cytokines, for example IL-6. It may play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2.
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Protein name
Mitogen-activated protein kinase
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Protein short names
EXIP; CSBP2; PRKM15; P38ALPHA; MXI2; RK; PRKM14; P38; RP1-179N16.5; P38A; MAPK14; CRK1; P38-ALPHA; MGC102436; P38MAPK; CSBP; CSBP1; CSPB1; RP24-137E7.1; SAPK2A
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Uniprot ID
Q16539
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Gene Name
MAPK14; hCG_1818055
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Source/Expression Host
Baculovirus-Insect Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the full length of human MAPK14 isoform 2 (NP_620581.1) (Met 1-Ser 360) was fused with a polyhistidine tag at the carboxy-terminus.
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Protein Species
Human
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Molecular weight
The recombinant human MAPK14 consists of 370 amino acids and predicts amolecular mass of 42.8 kDa which is also estimated by SDS-PAGE.
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Purity
> 95 % as determined by SDS-PAGE
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Activity
No Kinase Activity
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Validations
Human p38 alpha / MAPK14 Protein (His Tag) SDS-PAGE
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