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Product Name
MCM2 antibody
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Description
MCM2 Rabbit Polyclonal antibody. Positive WB detected in K-562 cells, HEK-293 cells, HeLa cells, PC-3 cells. Positive IP detected in HEK-293 cells. Positive IHC detected in human tonsillitis tissue, human placenta tissue. Observed molecular weight by Western-blot: 116-125 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
BM28 antibody; CCNL1 antibody; cdc19 antibody; CDCL1 antibody; D3S3194 antibody; KIAA0030 antibody; MCM2 antibody; MITOTIN antibody; Nuclear protein BM28 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MCM2 recombinant protein (Accession Number: NM_004526). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:112552(MCM2 antibody) at dilution of 1:1000
IP Result of anti-MCM2 (IP:Catalog No:112552, 4ug; Detection:Catalog No:112552 1:800) with HEK-293 cells lysate 2800ug.
Immunohistochemistry of paraffin-embedded human placenta tissue slide using Catalog No:112552(MCM2 Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:112552(MCM2 Antibody) at dilution of 1:50 (under 40x lens)
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Background
The MCM2-7 complex forms the core of the replicative helicase which acts as the molecular motor that uses ATP binding and hydrolysis to fuel the unwinding of double-stranded DNA at the replication fork in eukaryotes. This complex is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. MCM2, also named CDCL1 and BM28, is a human nuclear protein that plays an important role in 2 crucial steps of the cell cycle, namely, onset of DNA replication and cell division.
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References
- Cunha IW, Carvalho KC, Martins WK. Identification of genes associated with local aggressiveness and metastatic behavior in soft tissue tumors. Translational oncology. 3(1):23-32. 2010.
- Valle BL, D'Souza T, Becker KG. Non-steroidal anti-inflammatory drugs decrease E2F1 expression and inhibit cell growth in ovarian cancer cells. PloS one. 8(4):e61836. 2013.
- Liu YZ, Jiang YY, Wang BS. A panel of protein markers for the early detection of lung cancer with bronchial brushing specimens. Cancer cytopathology. 122(11):833-41. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"