-
Product Name
Anti-MCM2 (2B3) Mouse antibody
- Documents
-
Description
MCM2 (2B3) Mouse monoclonal antibody
-
Tested applications
WB, IHC-P, FC
-
Species reactivity
Human, Mouse, Rat, Monkey
-
Isotype
Mouse IgG1
-
Preparation
Antigen: Purified recombinant fragment of human MCM2 expressed in E. Coli.
-
Clonality
Monoclonal
-
Formulation
Ascitic fluid containing 0.03% sodium azide.
-
Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
-
Applications
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
FC: 1/200 - 1/400
ELISA: 1/10000
-
Validations
Western blot detection of MCM2 in K562 and Hela cell lysates using MCM2 mouse mAb (dilution 1:500).Predicted band size:125KDa.Observed band size:125KDa.
-
Background
Swiss-Prot Acc.P49736.The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"