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Product Name
Anti-Phospho-MCM2 (Ser108) Rabbit antibody
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Description
Phospho-MCM2 (Ser108) Rabbit monoclonal antibody
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Tested applications
WB, ICC/IF, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
BM28; CCNL1; CDCL1; cdc19; DFNA70; D3S3194; MITOTIN antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic phosphopeptide corresponding to residues surrounding Ser108 of human MCM2
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Clonality
Monoclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
1:1000
1:50
1:20
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Validations
Immunocytochemistry of MCM2 (Phospho-Ser108)(green) in Hela cells using MCM2 (Phospho-Ser108) Rabbit mAb at dilution 1/50, and DAPI(blue)
Western blot detection of Phospho-MCM2 (Ser108) in K562,3T3,Hela cell lysates using Phospho-MCM2 (Ser108) Rabbit mAb(1:1000 diluted).Predicted band size:102kDa.Observed band size:125kDa.
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Background
Swiss-Prot Acc.P49736.Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. Plays a role in terminally differentiated hair cells development of the cochlea and induces cells apoptosis.
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