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Product Name
KDM5C antibody
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Description
KDM5C Rabbit Polyclonal antibody. Positive WB detected in HeLa cells. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 171-180 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DXS1272E antibody; Histone demethylase JARID1C antibody; JARID1C antibody; KDM5C antibody; lysine (K) demethylase 5C antibody; Lysine specific demethylase 5C antibody; MRXJ antibody; MRXSJ antibody; Protein SmcX antibody; Protein Xe169 antibody; SMCX antibody; XE169 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of KDM5C recombinant protein (Accession Number: NM_001282622). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IF: 1:50-1:500
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112029(KDM5C antibody) at dilution of 1:500
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:112029(KDM5C Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
KDM5C belongs to the ARID protein family that harbors conserved DNA-binding motifs and acts as a histone H3 lysine 4 demethylase, suggesting a potential role in epigenetic regulation during development, cell growth and differentiation [PMID:21575681]KDM5C protein is homologous to three other proteins of the human JARID1 family (JARID1A, JARID1B and JARID1C). This family shares several evolutionarily conserved domains, including the catalytic JmjC domain, JmjN domain, Arid/Bright domain, C5HC2 zinc-finger domain and two plant homeodomains (PHD), a motif that binds methyl-lysine residues [PMID:17320160,17320161].
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