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Product Name
Anti-KDM5C/Jarid1C/SMCX (2E4) Mouse antibody
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Description
KDM5C/Jarid1C/SMCX (2E4) Mouse monoclonal antibody
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Tested applications
WB, ICC/IF
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Species reactivity
Human
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Alternative names
DXS1272E;Histone demethylase JARID1C;JARID1C;JmjC domain containing protein SMCX;Jumonji AT rich interactive domain 1C;Jumonji antibody; AT rich interactive domain 1C (RBP2 like); Jumonji/ARID domain-containing protein 1C;KDM5C;KDM5C_HUMAN;Lysine (K) specific deme antibody
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Isotype
Mouse IgG2a
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Preparation
Antigen: Purified recombinant human KDM5C / Jarid1C / SMCX protein fragments expressed in E.coli
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Clonality
Monoclonal
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Formulation
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4 150 mM NaCl) with 0.02% sodium azide 50% glycerol
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000
;ICC: 1/150
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Validations
Immunocytochemistry of HeLa cells using anti-KDM5C / Jarid1C / SMCX mouse mAb diluted 1:150.
Western blot detection of KDM5C / Jarid1C / SMCX in T47D,MCF7 and Hela cell lysates using KDM5C / Jarid1C / SMCX mouse mAb (1:500 diluted).Predicted band size: 176KDa.Observed band size: 220KDa.
Western blot detection of KDM5C / Jarid1C / SMCX in Hela and 293 cell lysates using KDM5C / Jarid1C / SMCX mouse mAb (1:1000 diluted).Predicted band size: 176KDa.Observed band size: 220KDa.
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Background
Swiss-Prot Acc.P41229.
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