Canine IL17 / IL17a recombinant protein

Canine IL17 / IL17a recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

IL17, also known as IL17a, is a cytokine belongs to the IL-17 family. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. The IL-17 family of cytokines includes six members, IL-17/IL-17A, IL-17B, IL-17C, IL-17D, IL-17E/IL-25, and IL-17F, which are produced by multiple cell types. IL-17 regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.

Interleukin 17A

CTLA8; CYTOTOXIC T-LYMPHOCYTE-ASSOCIATED ANTIGEN 8; CYTOTOXIC T-LYMPHOCYTE-ASSOCIATED PROTEIN 8; CYTOTOXIC T-LYMPHOCYTE-ASSOCIATED SERINE ESTERASE 8; IL-17; INTERLEUKIN-17A; CYTOTOXIC T-LYMPHOCYTE-ASSOCIATED ANTIGEN 8. INTERLEUKIN-17A; IL17; INTERLEUKIN 17 (CYTOTOXIC T-LYMPHOCYTE-ASSOCIATED SERINE ESTERASE 8); CTLA-8; IL-17A

C6L8D7

IL-17A; IL17A

E. coli

A DNA sequence encoding the canine IL17A (NP_001159350.1)(Gly26-Ala155) was expressed with a N-terminal Met.

Canine

The recombinant canine IL17A comprises 131 amino acids and has a predicted molecular mass of 15.2 kDa. The apparent molecular mass of the protein is approximately 15 kDa in SDS-PAGE under reducing conditions due to glycosylation.

> 95 % as determined by SDS-PAGE

1. Measured by its binding ability in a functional ELISA. Immobilized Canine IL17A (cat: 500487) at 10 μg/ml (100 μl/well) can bind human IL17Ra (Cat:503306), The EC₅₀ of human IL17Ra (Cat:503306) is 6.0-15.0 ng/ml.
2. Measured by its ability to induce IL-6 secretion by HFF human foreskin fibroblast cells. The ED₅₀ for this effect is typically 1-5 ng/mL.