Mouse IL10RB / IL-10RB (His Tag) recombinant protein

Mouse IL10RB / IL-10RB (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Interleukin 10 receptor, beta subunit (IL10RB/IL-10RB) also known as Cytokine receptor family 2 member 4, Interleukin-10 receptor subunit 2, and cytokine receptor family II, member 4, is a subunit for the interleukin-10 receptor. IL10RB/IL-10RB belongs to the cytokine receptor family. It is an accessory chain essential for the active interleukin 10 receptor complex. Coexpression of this and IL10RA proteins has been shown to be required for IL10-induced signal transduction. Defects in IL10RB/IL-10RB are the cause of inflammatory bowel disease type 25 (IBD25). It is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.

Interleukin 10 receptor 2

CDW210B; CRFB4; CRF2-4; 6620401D04RIK; IL10R2; D16H21S58; AI528744; IL-10R2; D21S58H; D21S58; D21S66; IL10RB

Q8VHM7

Il10rb; Il10r2; mCG_11439

Human Cells

A DNA sequence encoding the extracellular domain of mouse IL10RB (NP_032375.2) (Met 1-Ser 222) was expressed, with a C-terminal polyhistidine tag.

Mouse

The secreted recombinant mouse IL10RB comprises 212 amino acids and has a calculated molecular mass of 25 kDa. As a result of glycosylation, the apparent molecular mass of rmIL10RB is approximately 35-45 kDa in SDS-PAGE under reducing conditions.

> 98 % as determined by SDS-PAGE

Immobilized mouse IL10RB-His at 10 μg/ml (100 μl/well) can bind biotinylated human IL28B-His (Cat:500592), The EC₅₀ of biotinylated human IL28B-His (Cat:500592) is 0.13-0.31 μg/ml.