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Product Name
Rat IL-10 recombinant protein
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Description
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
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Protein name
Interleukin-10
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Protein short names
MGC126450; RP11-262N9.1; CSIF; TGIF; GVHDS; IL10; AL513351.1; MGC126451; IL10A; IL-10
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Uniprot ID
P29456
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Gene Name
Il10; Il-10
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
A DNA sequence encoding the rat IL10 (NP_036986.2) (Ser19-Asn178) was expressed and purified with an initial Met.
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Protein Species
Rat
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Molecular weight
The recombinant rat IL10 consists of 160 amino acids and has a predicted molecular mass of 18.6 kDa. The apparent molecular mass of the recombinant protein is approximately 18 kDa in SDS-PAGE under reducing conditions.
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Purity
> 80 % as determined by SDS-PAGE
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Activity
Measured in a cell proliferation assay using MC/9-2 mouse mast cells.
The ED50 for this effect is typically 2-8 ng/mL. -
Validations
Rat IL10 / Interleukin-10 Protein SDS-PAGE
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