Human IL-10 (His Tag) recombinant protein

Human IL-10 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

IL-10 is a anti-inflammatory cytokine which belongs to the IL-10 family. It is produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types, while it is produced primarily by monocytes and to a lesser extent by lymphocytes. IL-10 is mainly expressed in monocytes and Type 2 T helper cells (TH2), mast cells, CD4+CD25+Foxp3+ regulatory T cells, and also in a certain subset of activated T cells and B cells. IL-10 has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. The importance of interleukin 10 for counteracting excessive immunity in the human body is revealed by the fact that patients with Crohn's disease react favorably towards treatment with bacteria producing recombinant IL-10. IL-10 inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. It also displays a potent ability to suppress the antigen-presentation capacity of antigen presenting cells. However, it is also stimulatory towards certain T cells and mast cells and stimulates B cell maturation and antibody production.

Interleukin-10

IL10A; IL-10; MGC126451; AL513351.1; TGIF; GVHDS; IL10; CSIF; RP11-262N9.1; MGC126450

Q6FGW4

IL10

Human Cells

A DNA sequence encoding the human IL10 (NP_000563.1) (Ser 19-Asn 178) was expressed, with a N-terminal polyhistidine tag.

Human

The recombinant human IL10 comprises 176 amino acids and has a predicted molecular mass of 20.9 kDa as estimated in SDS-PAGE under reducing conditions.

> 85 % as determined by SDS-PAGE

1. Immobilized human IL10-His at 10 μg/ml (100 μl/well) can bind Cynomolgus IL10RA-Fc (Cat:504750), The EC₅₀ of Cynomolgus IL10RA-Fc (Cat:504750) is 0.31-0.71 μg/ml.
2. Measured in a cell proliferation assay using MC/9-2 mouse mast cells. The ED₅₀ for this effect is typically 5-20 ng/mL.